منابع مشابه
Oxidative modification of citrate synthase by peroxyl radicals and protection with novel antioxidants.
In mammals, aging is linked to a decline in the activity of citrate synthase (CS; E.C. 2.3.3.1), the first enzyme of the citric acid cycle. We used 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), a water-soluble generator of peroxyl and alkoxyl radicals, to investigate the susceptibility of CS to oxidative damage. Treatment of isolated mitochondria with AAPH for 8-24 h led to CS inactivat...
متن کاملPurification and characterization of mitochondrial citrate synthase.
Mitochondrial citrate synthase was purified from leaves of Pisum sativum L. cv Progress 9. A three step purification was employed using ATP-Sepharose affinity chromatography which resulted in a 600-fold enrichment. Enzyme activity was assayed spectrophotometrically during greening of etiolated leaves under constant white light illumination. An increase (1.4 fold) in citrate synthase activity wa...
متن کاملInteraction between Citrate Synthase and Malate Dehydrogenase
The interactions between pig heart citrate synthase and mitochondrial malate dehydrogenase or cytosolic malate dehydrogenase were studied using the frontal analysis method of gel filtration and by precipitation in polyethylene glycol. This method showed that an interaction between citrate synthase and mitochondrial malate dehydrogenase occurred but no interaction between citrate synthase and cy...
متن کاملRhizobium tropici chromosomal citrate synthase gene.
Two genes encoding citrate synthase, a key enzyme in the Krebs cycle, have been found in Rhizobium tropici. One of them is in the bacterial chromosome, while the other is in the symbiotic plasmid. We sequenced the chromosomal gene and found that it is very similar to the previously reported plasmidic gene sequence in its structural region but not in its regulatory region. The chromosomal gene i...
متن کاملCitrate synthase from blue-green bacteria.
Andrew, P. W., Delaney, M. E., Rogers, L. 3. &Smith, A. J. (1975)Phytochemistry in the press Bowyer, J. W. & Skerman, V. B. D. (1968) J. Gen. Microbiol. 54, 299-306 Boyer, P. D. (1954) J. Amer. Chem. SOC. 76,4331-4341 Buchanan, B. B. & Amon, D. I. (1970) Aduun. Enzymol. Relat. Areas Mol. BfoI. 33,119-176 Chung, A. E., Franzen, J. S . & Braginski, J. E. (1971) Biochemistry 10, 2872-2876 Delaney,...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1981
ISSN: 0014-5793
DOI: 10.1016/0014-5793(81)80351-1